FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l04n01_3008_d3.351000000be1eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l04n01_3008_d3.351000000be1eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14799466
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG317900.21480504769563982No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC202510.13683601827255118No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA182520.12332877415982442No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC172380.11647717559538974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT74650.046.1737671
AACCGTG74800.045.3933032
ACCGTGT100500.033.9742743
CCGTGTT115250.029.7085654
CGTGTTC125750.027.4545125
TTCACTA154400.022.0525679
TGTTCAC169250.020.650787
GTTCACT168000.020.2956288
GTGTTCA181550.019.5132646
ACAACCG31400.017.0897662
AACTCAC135400.016.5542935
ACTCACC137100.016.3836636
ATTAGGC42600.014.6032335
GAACTCA168650.014.3323954
TCACTAG145000.014.2137623
CACTAGC147750.013.5956574
AGACTCA105150.013.5487524
AGAACTC177800.013.3277283
CTTTATT193050.013.03921
ACTAGCA162500.012.5077095
Produced by FastQC (version 0.11.2)