FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l04n01_3002_d5.351000000be13e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l04n01_3002_d5.351000000be13e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13237418
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG178080.134527745516535No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC163050.1231735675340916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT51600.034.5537261
AACCGTG51900.033.4893722
ACCGTGT70900.025.251573
CCGTGTT80900.021.8954374
CGTGTTC89750.019.9480385
TTCACTA105850.016.8241739
AACTCAC110550.016.323685
ACTCACC115100.015.4720976
GTTCACT119200.015.0992798
TGTTCAC126650.014.9235157
GTGTTCA131950.014.1441366
CGCGTAT4102.5362278E-612.7903331
GAACTCA144750.012.6309344
CACTAGC92350.012.5471824
CTCGGTT33100.012.38635351
ACAACCG26650.012.1172742
TCACTAG92950.012.1085523
AGAACTC153350.011.4580643
TAGCAAC101300.011.3917417
ACTAGCA101700.011.3469355
Produced by FastQC (version 0.11.2)