FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l03n02_3032_d8.352000000be4a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l03n02_3032_d8.352000000be4a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14249132
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG487580.3421822466098286No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA330140.2316913058283129No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC273850.19218714515382412No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT270460.18980805286946603No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC262140.18396910071434525No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC211110.14815639296484867No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG204370.14342628028149365No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG201490.1414051045354903No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA200950.14102613408311468No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA187040.13126413594877218No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT180500.12667438269222292No Hit
CTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGA176920.12416194895239933No Hit
CTTGAAGTTCTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTGA175400.12309521730867537No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG174890.1227373007703206No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC172220.12086350242246334No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA166230.11665973758963002No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC159160.11169803185204544No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC157820.11075762369244667No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA154630.10851889083489435No Hit
CCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGG151260.10615383449321684No Hit
CGACAAACTGCACGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGC144130.10115002092759054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT98200.053.3995321
AACCGTG106300.049.1329422
ACCGTGT131250.039.935173
CCGTGTT144050.036.6791154
CGTGTTC162300.032.4821975
ACAACCG39250.032.0859262
TGTTCAC208250.025.7424017
GTGTTCA212150.025.2020286
TTCACTA217500.024.3167179
GTTCACT222950.023.7653068
ATTAGGC56850.022.3895475
TATTAGG62150.020.4890144
AGACTCA105200.018.7890554
AACTCAC161500.018.3213085
ACTCACC165650.017.9441576
TTATTAG73550.017.8968433
CTCAGAA183000.017.4319061
ACAGACT102750.017.2518372
CACAACC79750.017.2282281
TTAGGCA74300.016.9354696
Produced by FastQC (version 0.11.2)