FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l03n02_3028_d3.352000000be339.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l03n02_3028_d3.352000000be339.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12923628
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG251820.19485240522243444No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC232830.18015838896012792No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC193360.14961742940914113No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA167190.12936769767746334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT57350.044.8482321
AACCGTG61100.042.0010762
ACCGTGT80800.031.816943
CCGTGTT89750.029.011584
CGTGTTC99550.026.1928565
TTCACTA131250.020.111629
ACAACCG25300.019.7231662
TGTTCAC136850.019.6733767
AACTCAC133500.019.3183635
GTTCACT139950.018.7939728
ACTCACC137200.018.6541426
GTGTTCA147700.018.4532156
GAACTCA165150.016.0539134
AGACTCA94250.015.0735354
AGAACTC172650.015.0279083
ATTAGGC36550.014.8138945
CACTAGC120000.014.4918944
TCACTAG121650.014.4140733
TATTAGG40800.013.8583694
CTTTATT178000.013.8353841
Produced by FastQC (version 0.11.2)