FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l03n02_3027_d5.352000000be1f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l03n02_3027_d5.352000000be1f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15017386
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC177650.11829622012779055No Hit
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG161410.10748208776147859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT49700.034.721241
AACCGTG54500.031.3033372
ACCGTGT70900.024.1945423
CCGTGTT84500.020.4102544
CGTGTTC97450.017.9836465
AACTCAC125150.016.7355885
TTCACTA106150.016.3698089
ACTCACC130750.015.470546
GTTCACT118450.014.8706538
TGTTCAC129900.014.4751677
GTGTTCA139950.013.5035436
GAACTCA160050.013.4177974
AGAACTC169100.012.1112813
GCGCGAA35700.011.97076814-15
CACTAGC93200.011.9288454
TCACTAG94550.011.659583
CGACAAT13650.011.4928121
CGCGGTT16100.011.2202551
CGGAAAT28850.011.2049031
ACTAGCA99050.011.1720955
Produced by FastQC (version 0.11.2)