FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l03n02_3027_d3.352000000be42b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l03n02_3027_d3.352000000be42b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13566120
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG336630.24814021990075277No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC247360.18233658555283308No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA233860.17238532461750303No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC233660.17223789852957222No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT184490.1359931948117811No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG151010.11131406769216254No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC145220.10704608244656542No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG143570.10582981722113617No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA140570.10361842590217395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT68400.049.3476871
AACCGTG77450.043.504932
ACCGTGT99750.033.9668243
CCGTGTT111950.030.388854
CGTGTTC121000.028.2617575
ACAACCG34650.022.9048182
TGTTCAC161600.021.4495417
TTCACTA163700.020.7941539
GTTCACT169200.020.258968
GTGTTCA174600.020.1788396
AACTCAC148500.017.974715
ACTCACC153700.017.3310766
ATTAGGC40350.016.950055
AGACTCA97700.016.8270074
GAACTCA177450.016.1192874
CACTAGC151350.015.6340774
TCACTAG151550.015.6153493
AGAACTC180150.015.4839633
ACAGACT99500.015.1885972
CTCAGAA175350.014.8030571
Produced by FastQC (version 0.11.2)