FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l03n02_3004_d5.352000000be0a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l03n02_3004_d5.352000000be0a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14240949
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG202240.14201300770054018No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC177900.12492145010841622No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC176270.12377686346605131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT55000.034.5747341
AACCGTG59500.031.8675942
ACCGTGT76700.025.028863
CCGTGTT90200.021.3859564
CGTGTTC97400.020.333785
TTCACTA113650.017.2526829
ACAACCG29650.016.3481562
AACTCAC131650.015.9456585
ACTCACC134650.015.5511766
GTTCACT128050.015.312788
TGTTCAC134850.015.1407887
GTGTTCA142050.014.4067836
CGCGTAT4301.9845174E-814.3726381
CGCGATT4856.508344E-913.7229621
GAACTCA166650.013.5709684
AGACTCA100650.013.2176254
CTCAGAA183050.012.9336531
AGAACTC170950.012.5638993
TCACTAG100900.012.338583
CTTTATT169950.012.0284281
Produced by FastQC (version 0.11.2)