FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l03n01_3028_d3.351000000be33c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l03n01_3028_d3.351000000be33c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12923628
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG259970.2011586839237403No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC224960.174068767686597No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC187140.14480453940642674No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA180030.13930298829399917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT60450.041.9510881
AACCGTG64000.039.7720682
ACCGTGT83600.030.6747363
CCGTGTT94000.027.6850974
CGTGTTC102200.025.5567235
TTCACTA129050.020.3130179
TGTTCAC139950.019.3077967
GTTCACT138700.019.0024628
GTGTTCA149600.018.2528046
ACAACCG28100.017.5760142
AACTCAC132800.017.415035
ACTCACC132650.017.3273246
TCACTAG119850.016.047613
CACTAGC121350.015.5361744
ATTAGGC36950.014.5230335
GAACTCA165900.014.3411734
ACTAGCA131350.014.3172125
AGACTCA94250.013.8562184
CGGCAAT19000.013.7986631
TAGCAAC137350.013.6917787
Produced by FastQC (version 0.11.2)