FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l03n01_3027_d5.351000000be1f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l03n01_3027_d5.351000000be1f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15017386
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC169680.11298903817215593No Hit
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG169030.1125562065195634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT49150.035.975921
AACCGTG56700.030.9057252
ACCGTGT75100.023.9027293
CCGTGTT87050.020.7305344
CGTGTTC94850.019.1759595
CGTATGC32850.018.1357781
TATGCCG33000.017.9883653
TTCACTA108650.016.609239
ACTCACC122450.016.5601676
AACTCAC121950.016.550185
GTTCACT118400.015.1612758
TGTTCAC132050.014.6369857
GAACTCA161550.014.0219044
GTGTTCA143950.013.295026
CGACAAT13000.013.1983821
AGAACTC162700.012.8720193
CTCGAAT26000.012.831761
ATTAGGC36850.012.7583155
ACAACCG27950.012.2333762
CGACGAT11050.011.645631
Produced by FastQC (version 0.11.2)