FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l03n01_3027_d3.351000000be42e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l03n01_3027_d3.351000000be42e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13566120
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG358840.26451188696546984No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA247070.1821228177253334No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC244800.18044953162731864No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC223910.1650508767429449No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT179120.1320348043508387No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG162580.11984266687896022No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG145890.10753995984113364No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC141690.10444401199458651No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA139670.10295500850648527No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA138390.10201148154372805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT73300.049.9369471
AACCGTG79050.046.4384042
ACCGTGT102300.036.1163333
CCGTGTT115750.032.0017174
CGTGTTC127900.029.1473335
ACAACCG34500.023.5383872
TGTTCAC169100.022.607477
TTCACTA167550.022.1363669
GTGTTCA176500.021.740346
GTTCACT174800.021.4355818
AACTCAC145050.018.596465
ACTCACC149150.017.8305386
ATTAGGC43400.016.8511945
AGACTCA94650.016.1058674
TCACTAG158350.015.9548683
GAACTCA175750.015.8073914
CACTAGC159600.015.6513764
TATTAGG47100.015.6282544
CTCAGAA173500.015.4109051
AGAACTC178050.015.2564593
Produced by FastQC (version 0.11.2)