FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l03n01_3004_d5.351000000be0a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l03n01_3004_d5.351000000be0a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14240949
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG216000.1516752851232035No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC168350.1182154363448672No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC168290.11817330432122185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT57300.038.264261
AACCGTG59900.036.152972
ACCGTGT79800.027.077873
CCGTGTT90850.023.9935074
CGTGTTC99600.022.4101335
TTCACTA117100.018.8582769
ACAACCG30750.017.4517612
GTTCACT133500.016.7550458
TGTTCAC138650.016.4409667
AACTCAC123800.015.7662045
GTGTTCA146650.015.6088516
ACTCACC128250.014.9969736
AGACTCA101550.014.8246784
CGCGTAT3951.6727754E-613.2735061
GAACTCA160750.013.0875624
ACAGACT103050.013.04200652
TCACTAG107400.012.734863
CACTAGC108100.012.6963264
ACTAGCA109600.012.5658945
CTCAGAA186900.012.4196611
Produced by FastQC (version 0.11.2)