FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l02n02_3034_d5.352000000bdfb6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l02n02_3034_d5.352000000bdfb6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12618447
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG318260.2522180423628993No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC216080.17124135798961632No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA201900.16000384199418516No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC201060.1593381499323966No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT187900.14890897429770875No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC152190.12060913676619635No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA148030.11731237607924334No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC138260.1095697434082023No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG128610.10192220960313103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT71850.044.4317931
AACCGTG75550.042.171672
ACCGTGT95150.033.4819073
CCGTGTT104300.030.4495684
CGTGTTC113850.027.9676745
ACAACCG33750.024.7981552
TTCACTA149150.021.576949
TGTTCAC151050.021.3083237
GTTCACT157250.020.3761628
GTGTTCA160300.020.3450286
AGACTCA97500.018.6272054
ATTAGGC43950.017.8418435
ACAGACT97450.017.0791662
AACTCAC137350.016.5045745
TATTAGG47950.016.459194
ACTCACC142200.016.4582046
TCACTAG137350.014.8862063
GAACTCA161600.014.5924854
CTCAGAA169350.014.5807631
CTTTATT195150.014.4815821
Produced by FastQC (version 0.11.2)