FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l02n02_3030_d3.352000000be210.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l02n02_3030_d3.352000000be210.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13992666
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG211220.1509505050717283No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC190750.1363214129458961No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC146050.10437610674048821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT59150.038.769231
AACCGTG60950.037.5271072
ACCGTGT77050.030.1140753
CCGTGTT87950.026.3244574
CGTGTTC97250.023.847055
TTCACTA113950.020.4521129
GTTCACT127650.018.1093338
TGTTCAC135550.017.6153937
AACTCAC131900.017.1140695
GTGTTCA145100.016.9140266
ACAACCG28250.016.3277742
ACTCACC135500.015.9753136
GAACTCA163950.014.3245794
AGAACTC164700.014.0591053
ATTAGGC39900.013.1015985
TCACTAG106250.013.0670113
CACTAGC106200.012.7581194
AGACTCA97950.012.6192984
ACAGACT97350.011.820972
CGACGAT8501.8189894E-1211.7542591
Produced by FastQC (version 0.11.2)