FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l02n02_3022_d8.352000000be587.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l02n02_3022_d8.352000000be587.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15393791
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG438270.2847056972515737No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA271180.1761619343799068No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT242350.15743360423692904No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC234730.15248355651963835No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC220160.1430187015011442No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA178390.11588438481463079No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC165060.10722504937217868No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC161700.10504235116612926No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG155400.10094979202978654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT91550.047.4994741
AACCGTG100500.043.2481272
ACCGTGT123700.035.325223
CCGTGTT139750.031.4341094
ACAACCG44500.029.1735822
CGTGTTC153300.028.6446745
TGTTCAC196100.022.6592627
TTCACTA193000.022.5521269
GTGTTCA209100.021.636266
GTTCACT203350.021.522328
AGACTCA114100.017.7502694
AACTCAC156600.017.662225
ACTCACC163700.016.587876
ACAGACT115450.016.1053432
TCACTAG184450.015.5960913
CACTAGC185450.015.2791944
GAACTCA188350.015.1701484
ATTAGGC50000.015.1126695
CACAACC92700.015.0379551
ACTAGCA193350.014.6246955
Produced by FastQC (version 0.11.2)