FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l02n02_3016_d3.352000000be302.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l02n02_3016_d3.352000000be302.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13308054
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG548150.41189342934737116No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC366810.27563008085179097No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC343850.2583773705757431No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA286730.21545599379142888No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT227950.1712872520655537No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC209770.1576263516814705No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG201360.15130687026067072No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT200100.15036007518454614No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA178890.13442235806978242No Hit
CCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGG172390.12953809775644132No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC160150.12034065987408828No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA159970.12020540343464191No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA154270.11592228285217357No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT142350.10696530086216963No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG141340.10620636195194277No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC140570.10562776496097777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT98050.056.7224351
AACCGTG103650.053.5858652
ACCGTGT130150.042.707213
CCGTGTT147250.037.7752574
CGTGTTC157500.035.3947535
ACAACCG37200.030.934932
TGTTCAC202200.027.8448037
TTCACTA209500.026.9158239
GTGTTCA210300.026.8848866
GTTCACT215500.025.9921768
ACAGACT105150.022.2953552
AGACTCA119400.021.143024
AACTCAC202750.018.7521255
ACTCACC207800.018.3901826
CACAGAC143100.018.2191831
CTCAGAA205450.018.1087551
CAGACTC151300.017.2529873
GAACTCA228150.017.0871734
CACAACC74250.016.9159131
AGAACTC231950.016.5633373
Produced by FastQC (version 0.11.2)