FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l02n02_3011_d3.352000000bdfd0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l02n02_3011_d3.352000000bdfd0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11762016
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG194630.1654733338230453No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA135570.11526085324148515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT47150.038.951721
AACCGTG53350.034.228962
ACCGTGT66350.027.6633223
CCGTGTT76900.024.2360634
CGTGTTC83600.022.1714485
TTCACTA104350.017.739599
ACAACCG25850.017.4767192
TGTTCAC118550.016.2177127
GTTCACT112550.016.1952848
GTGTTCA126750.015.3930426
TCACTAG96300.015.0108483
AACTCAC91950.014.627515
CACTAGC95650.014.5641454
ACTCACC94550.013.8070776
ACTAGCA104700.013.3455485
ATTAGGC35400.012.7542845
CTTTATT155450.012.6103621
TAGCAAC110800.012.4677867
GAACTCA121500.012.3655424
CTAGCAA115700.011.9396126
Produced by FastQC (version 0.11.2)