FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l02n01_3034_d5.351000000bdfb9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l02n01_3034_d5.351000000bdfb9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12618447
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG326270.2585658916663834No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA213490.16918880746576817No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC199320.15795921637583454No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT184110.14590543511416262No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC182150.1443521536366559No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA147010.11650403571850007No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC141830.11239893467080378No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC138600.10983919019511672No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG128060.10148633980076947No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT126660.10037685303112183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT72250.046.072531
AACCGTG77600.042.472022
ACCGTGT98900.033.18083
CCGTGTT109900.029.989344
ACAACCG34800.028.5214862
CGTGTTC120150.027.5495265
TTCACTA149400.021.933289
TGTTCAC155450.021.7823097
GTGTTCA163100.020.6732866
GTTCACT163150.020.4049768
AGACTCA93950.017.6919774
ATTAGGC41100.017.4477845
AACTCAC128800.017.218935
ACTCACC133900.016.8113616
ACAGACT91450.016.669652
CACAACC66450.016.0759811
TATTAGG45650.016.0208324
CACTAGC143050.014.6404984
TCACTAG144400.014.5036243
GAACTCA161200.014.3767224
Produced by FastQC (version 0.11.2)