FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l02n01_3030_d3.351000000be213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l02n01_3030_d3.351000000be213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13992666
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG215490.15400210367345293No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC191710.13700748663621357No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC143580.10261089630810884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT60950.037.474111
AACCGTG61350.036.7679672
ACCGTGT81600.027.7599583
CCGTGTT92600.024.7187564
CGTGTTC103550.022.288295
TTCACTA120900.019.0112049
ACAACCG29550.018.3205262
AACTCAC129100.017.4358315
TGTTCAC138350.017.2998357
ACTCACC132950.017.1809566
GTTCACT137000.017.0196848
GTGTTCA150350.015.7295566
GAACTCA166550.013.9429554
AGAACTC166650.013.84913
CGCATAT5351.720764E-913.3692481
AGACTCA103800.013.1303134
CGCGTAT4102.5299469E-612.7931761
CACTAGC109600.012.69543654
TCACTAG111800.012.4880913
ACAGACT99700.012.288962
Produced by FastQC (version 0.11.2)