FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l02n01_3022_d8.351000000be58a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l02n01_3022_d8.351000000be58a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15393791
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG490290.31849854269166056No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA291710.1894984802638934No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT231620.15046326145391997No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC218970.14224566255316834No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC203430.1321506833501897No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT184330.11974308342889675No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA176580.1147085860786339No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG174180.11314951593145574No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC168150.1092323521866706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT101450.047.4716721
AACCGTG110400.043.4029242
ACCGTGT135650.035.603943
CCGTGTT154350.031.3827084
CGTGTTC168550.028.964185
ACAACCG47300.028.413332
TGTTCAC216750.022.8299797
TTCACTA216950.022.2616929
GTGTTCA224850.022.1342746
GTTCACT224500.021.6822538
AGACTCA114700.017.844764
AACTCAC154600.016.1574615
ACAGACT116650.016.121572
ACTCACC158150.015.7647486
CACAACC90200.015.59483051
CTTTATT225600.014.858721
ATTAGGC55900.014.3572675
CACTAGC203800.014.1909034
TCACTAG204350.014.1527073
GAACTCA187950.013.7705434
Produced by FastQC (version 0.11.2)