FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l02n01_3016_d3.351000000be305.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l02n01_3016_d3.351000000be305.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13308054
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG571370.4293415100359526No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC350010.2630061465034632No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC325010.24422052991368987No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA302740.22748630265551975No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT225680.16958151807920227No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT222410.1671243594292599No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG214560.16122567582007105No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC200070.15033753244463843No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA174070.1308004911912741No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC170680.12825316158170083No Hit
CCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGG169220.12715608157285804No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA168080.1262994574563644No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA153210.11512577270876719No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC146170.109835743077087No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG142140.10680750168281554No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT133790.10053310574183122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT97050.058.523061
AACCGTG106000.053.806082
ACCGTGT137250.041.555153
CCGTGTT150800.037.852744
CGTGTTC168950.033.786295
ACAACCG33900.031.7995052
TGTTCAC211600.027.672077
GTGTTCA216600.026.879816
TTCACTA219500.026.0054389
GTTCACT228850.025.0674518
ACAGACT101800.020.805652
AGACTCA112450.020.1443444
CTCAGAA201200.018.7481881
ATTAGGC48400.018.0537195
AACTCAC196700.017.9623345
ACTCACC199150.017.550596
CACAACC72250.016.6991161
TCAGAAA216750.016.6732312
TATTAGG52800.016.6391834
CACAGAC140700.016.5739331
Produced by FastQC (version 0.11.2)