FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l02n01_3011_d3.351000000bdfd3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l02n01_3011_d3.351000000bdfd3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11762016
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG206310.17540360428008261No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA141680.12045554095488392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT51950.039.8388751
AACCGTG54800.037.263312
ACCGTGT73850.027.7153473
CCGTGTT79050.026.012354
CGTGTTC86950.024.3043485
ACAACCG26600.021.0665742
TTCACTA106000.019.6676379
GTTCACT117000.017.8185448
TGTTCAC126200.017.385067
GTGTTCA127900.017.2282436
TCACTAG99000.014.5345313
AACTCAC87500.013.8396675
CACTAGC100200.013.8391284
ACTCACC88050.013.69928556
ACTAGCA106950.013.1433025
ATTAGGC33500.012.4747375
TAGCAAC115550.012.3294837
CGACGCT17500.011.9899531
CTAGCAA118500.011.8622466
CTTTATT151150.011.7049251
Produced by FastQC (version 0.11.2)