FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l01n02_3035_d5.352000000be4d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l01n02_3035_d5.352000000be4d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15531983
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG324130.2086855232844383No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC267310.17210294397051557No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC224570.144585530385914No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA182880.11774414123425193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT75400.042.9952851
AACCGTG77400.041.3893172
ACCGTGT100000.032.176363
CCGTGTT114600.028.076654
CGTGTTC129350.025.0895655
ACAACCG35400.020.6769642
TTCACTA157650.020.6592399
TGTTCAC169200.019.9508697
GTTCACT172000.019.3207048
GTGTTCA186600.018.3194926
AACTCAC167550.017.8095955
ACTCACC169600.017.272376
AGACTCA119000.015.8956164
GAACTCA200700.015.5108354
ACAGACT116850.014.7247922
AGAACTC205150.014.4564393
ATTAGGC47750.014.4289075
CTCAGAA201250.013.4514651
CTTTATT213800.012.9968391
TCACTAG143600.012.974183
Produced by FastQC (version 0.11.2)