FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l01n02_3012_d8.352000000be1db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l01n02_3012_d8.352000000be1db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16556001
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG530480.3204155399604047No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC388690.23477287782236786No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC356880.21555930082391273No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA342380.2068011472094016No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT294320.17777239805675296No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC256490.15492267728179046No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA229380.13854795007562515No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC210130.126920746139119No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC203290.12278931367544614No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG200840.12130948771989082No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG190220.11489489521050403No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT187630.11333050777177411No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA184810.11162719789640022No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG184530.11145807493005105No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA181330.10952524102891756No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC176830.10680719335544858No Hit
CTTGAAGTTCTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTGA170720.10311668862547181No Hit
CTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGA168900.10201738934420214No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA167400.10111137345304583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT101700.050.0915371
AACCGTG113500.044.8068662
ACCGTGT142550.035.574013
CCGTGTT153900.032.826534
CGTGTTC170550.029.7827115
ACAACCG48350.028.507442
TGTTCAC221850.023.4774027
TTCACTA222200.023.0979799
GTGTTCA227450.022.8367466
GTTCACT235600.021.742118
ACAGACT129400.021.0463622
AGACTCA136200.020.6573684
AACTCAC222100.017.9280785
ATTAGGC57500.017.4362415
ACTCACC231200.017.1680626
CTCAGAA234300.016.7094611
CACAGAC172850.016.6283051
CAGACTC176150.016.6201023
TATTAGG63550.016.0787874
TCACTAG211550.015.9507453
Produced by FastQC (version 0.11.2)