FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l01n02_3009_d5.352000000be155.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l01n02_3009_d5.352000000be155.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14948868
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT48450.029.2685741
AACCGTG50600.027.522232
ACCGTGT68100.020.6583253
CCGTGTT77550.018.5084384
CGTGTTC84300.017.1917175
AACTCAC115150.014.40152555
TTCACTA100350.014.2405769
ACTCACC122350.013.31024556
GTTCACT113000.012.7295648
ACAACCG27950.012.5839342
AGACTCA105850.012.4375134
CGACAAT13450.012.0696731
TGTTCAC132000.011.7976597
CGATCTA7752.5465852E-1111.6518954
CACTAGC82700.011.4939464
TCACTAG85200.011.4915363
CGACGAT10100.011.34563351
GTGTTCA137900.011.2929016
GAACTCA155600.011.0877064
AGAACTC153450.010.8405573
Produced by FastQC (version 0.11.2)