FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l01n02_3005_d8.352000000be6e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l01n02_3005_d8.352000000be6e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13061844
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG997270.7634986300556033No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC584070.4471573845163056No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA564490.43216715802148603No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC468400.3586017410711688No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT431460.3303208949670506No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT387120.29637469257786264No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC383220.2933888966978935No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG373200.28571769805243424No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG353850.27090355695566415No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA343830.2632323583102049No Hit
CCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGG342010.2618389868995526No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA337850.25865413796091885No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC331700.2539457675348136No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG296640.22710422816257797No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC292840.22419499115132593No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA281090.21519932407705988No Hit
CTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGA271660.20797982275703183No Hit
CTTGAAGTTCTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTGA270980.20745922244975518No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA265750.2034551936158478No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA262200.2007373537763887No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA261910.20051533305710892No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC257180.19689409856678736No Hit
CCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCA253810.19431406469101914No Hit
CGACAAACTGCACGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGC253210.19385471147871616No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC239830.18361113484436042No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA236240.18086267145741444No Hit
GAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCA231120.17694285737909593No Hit
GTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAG228450.17489873558434782No Hit
CAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGG227990.17454656478824887No Hit
CGACCAGCAGCCTGCCCAGGGTCTCACCACCAACTTCGTCCACGTTCACC224280.1717062307588423No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT221490.16957023832163362No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC218820.16752611652688548No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC207150.15859169654759314No Hit
CTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGT204010.15618774806987434No Hit
CGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGA203860.1560729097667986No Hit
CCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACC203700.1559504155768512No Hit
GTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCC203200.1555676212332654No Hit
CAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCAC202700.15518482688967958No Hit
CTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGG201300.15411300272763936No Hit
CTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAG199680.15287274905442141No Hit
GGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACA193810.14837874346072422No Hit
CTGGTGTGCGTGCTGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGT192370.14727629575119716No Hit
CTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTT190920.14616619215479834No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT190680.14598245086987718No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA190520.14585995667992974No Hit
CTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCAC189440.14503312089778442No Hit
CCGGTCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT188250.14412207036005023No Hit
CTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATG187880.14383880254579673No Hit
AAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACAC187600.1436244377133887No Hit
CGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGC186870.14306555797175344No Hit
CTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTGAGCTTAGCGA186700.14293540789493428No Hit
CTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCT183270.14030943869793575No Hit
CTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAG179960.13777534014339782No Hit
CTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACCT173020.13246215465442707No Hit
CCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTG171290.13113768622562022No Hit
CTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGA169580.1298285295705568No Hit
CCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCAC167970.12859593178421055No Hit
CCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCA165200.12647525112074529No Hit
TTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAG162650.12452299996845775No Hit
CAGCAGCCTGCCCAGGGTCTCACCACCAACTTCGTCCACGTTCACCTTGC162530.12443112932599715No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC161230.12343586403267409No Hit
GTCGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCA160500.12287698429103884No Hit
TGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTC159230.12190468665833093No Hit
CAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCC158880.12163673061782088No Hit
CTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGCT158170.12109316264992906No Hit
GGACAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTAGACGA153480.11750255170709435No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC153330.11738771340401861No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC151680.11612449207018549No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGCCACCACCTTC150410.11515219443747758No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG148740.11387366132990105No Hit
AGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCA147790.11314635207708805No Hit
GTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTC143570.10991556781722396No Hit
CTTGAAGTTGACGGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCA143490.10985432072225024No Hit
AGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCAC142370.10899686139261806No Hit
GGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCT142330.10896623784513121No Hit
CCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGC141960.10868297003087772No Hit
CTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCACCTTCGCTA139890.10709820144843255No Hit
AGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGC137180.10502345610619757No Hit
GTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTC137180.10502345610619757No Hit
CAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGC136450.10446457636456231No Hit
GCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGG135910.10405115847348965No Hit
CTTGACCTGGGCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCT134620.10306354906703832No Hit
TTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCCA131530.10069788002367813No Hit
TGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTG131120.10038398866193778No Hit
CCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACT130680.10004712963958227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT151500.065.110741
AACCGTG165700.059.4040532
ACAACCG46900.048.4410482
ACCGTGT211650.046.3024373
CCGTGTT236000.041.6047974
CGTGTTC257700.038.12955
ACAGACT84650.034.1939132
CACAACC71900.032.6716581
GTGTTCA304300.032.4838186
TGTTCAC304600.032.467417
GTTCACT350850.028.1304248
CACAGAC110150.027.6981831
TTCACTA356050.027.5620219
AGACTCA113450.026.8102324
CTCAGAA194800.025.3434961
TCAGAAA201700.023.8940932
ATTAGGC81600.023.4078185
TATTAGG83500.023.1081224
CAGACTC134900.022.8999673
AACTCAC265400.022.7546255
Produced by FastQC (version 0.11.2)