FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l01n02_3003_d3.352000000be46e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l01n02_3003_d3.352000000be46e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15117654
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG395000.26128392672566786No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA280990.18586878625479852No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC230830.15268903495211625No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC216270.14305791096951948No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT195780.12950422069456013No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC162790.10768205172575057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT80200.047.789831
AACCGTG88450.043.579142
ACCGTGT113450.034.0168573
CCGTGTT124400.031.0987074
CGTGTTC138250.028.114385
ACAACCG37250.026.0291232
TGTTCAC182050.021.7507557
TTCACTA184700.020.924089
GTGTTCA194450.020.6323056
GTTCACT191100.020.4707558
ATTAGGC45750.017.6562375
AACTCAC149950.017.0164185
TCACTAG174150.016.7293723
CACTAGC176550.016.5017934
ACTCACC154550.016.2204886
AGACTCA104750.015.9708124
TATTAGG48850.015.8584744
ACTAGCA184500.015.7614225
GAACTCA185950.014.3641014
TAGCAAC202950.014.3640967
Produced by FastQC (version 0.11.2)