FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l01n01_3035_d5.351000000be4db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l01n01_3035_d5.351000000be4db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15531983
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG338950.21822712528078353No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC261830.16857474026336494No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC217080.13976322276427935No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA194800.12541862813009774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT73900.043.746181
AACCGTG77200.041.7189752
ACCGTGT103750.031.2260783
CCGTGTT117450.027.7454814
CGTGTTC130800.025.2405175
ACAACCG34250.021.636412
TTCACTA162000.020.1972669
TGTTCAC173850.019.5581027
GTTCACT174200.019.055378
GTGTTCA186650.018.1962746
AACTCAC161850.016.9349635
ACTCACC166200.016.291026
AGACTCA117200.016.2126334
ATTAGGC48050.015.6201385
GAACTCA197750.014.653274
ACAGACT115150.014.603622
TATTAGG53450.013.9531784
AGAACTC202850.013.9101753
CACTAGC148850.013.4674334
TCACTAG151050.013.3341813
Produced by FastQC (version 0.11.2)