FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l01n01_3012_d8.351000000be1de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l01n01_3012_d8.351000000be1de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16556001
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG559300.3378231252824882No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA368500.22257790392740373No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC368120.2223483799016441No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC336060.20298380025466295No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT292000.17637109347843116No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC248580.1501449534824261No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA231720.139961334865829No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT206760.12488523043698778No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG206410.12467382672905129No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC206020.12443826259735066No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG205300.12400337496959561No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA203500.12291615590020802No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC197610.1193585335009342No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG194240.11732301779880298No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC181000.10932591753286316No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA177020.10692195536832838No Hit
CTTGAAGTTCTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTGA174020.10510992358601573No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC171100.10334621265123142No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA167760.10132881726692333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT109050.051.421011
AACCGTG121450.045.800892
ACCGTGT154700.036.018223
ACAACCG47950.033.484412
CCGTGTT168250.033.173964
CGTGTTC187200.029.9680585
TGTTCAC234050.024.5710727
GTGTTCA242500.023.583936
TTCACTA246500.022.9062259
GTTCACT253750.022.3640528
AGACTCA135800.021.617394
ACAGACT128750.021.177722
ATTAGGC59050.019.6283825
TATTAGG64500.017.7489174
AACTCAC215600.017.6260815
CACAGAC169900.017.5967751
CACAACC98200.017.3346731
ACTCACC222400.017.1079966
CAGACTC177450.016.9985663
TCACTAG233350.016.0410563
Produced by FastQC (version 0.11.2)