FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l01n01_3009_d5.351000000be158.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l01n01_3009_d5.351000000be158.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14948868
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT49450.030.1815281
AACCGTG56400.026.278082
ACCGTGT72650.020.2695563
CCGTGTT81300.018.288244
CGTGTTC91350.016.5882385
ACAACCG29400.016.1573752
TTCACTA102450.014.5549079
AACTCAC115300.013.513345
GTTCACT117350.012.9496678
CGCGGTT14750.012.9309711
TCACTAG89600.012.8829813
AGACTCA106800.012.7207564
CACTAGC87250.012.5766434
ACTCACC118700.012.4453196
TGTTCAC131050.011.9945157
ACTAGCA96150.011.5113125
GTGTTCA140550.011.3892576
TAGCAAC98500.011.0887697
GAACTCA152800.011.0673794
CGGGGAT43450.010.7547291
Produced by FastQC (version 0.11.2)