FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l01n01_3003_d3.351000000be461.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l01n01_3003_d3.351000000be461.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15117654
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG418900.2770932579883096No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA302820.2003088574457386No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC230430.15242444363391303No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC215220.14236335875923606No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT196170.12976219722980828No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC169700.11225286674771098No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT161370.10674275254612918No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG158090.10457310373686289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT85200.047.6723141
AACCGTG91350.044.148422
ACCGTGT116700.034.8839763
CCGTGTT132550.030.9993364
CGTGTTC145750.028.2896255
ACAACCG37300.024.4516622
TGTTCAC189450.021.9081867
TTCACTA192450.021.1965319
GTTCACT196000.020.8610738
GTGTTCA201500.020.6743536
ATTAGGC50650.017.9130655
AACTCAC144300.017.4142725
TATTAGG54350.016.6935924
ACTCACC149450.016.4960066
AGACTCA102000.015.9272914
CACTAGC181350.015.9258284
TCACTAG184400.015.7654543
ACTAGCA190750.015.165925
CTTTATT227700.014.6764561
GAACTCA179200.014.4203854
Produced by FastQC (version 0.11.2)