FastQCFastQC Report
Tue 5 Dec 2017
CBN9DACXX_l08n03.333000000aae3b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBN9DACXX_l08n03.333000000aae3b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224126693
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5391040.24053538326200175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT19557400.084.1387251
AGCAGTG21850050.075.293282
GCAGTGG28289000.058.2227753
CAGTGGT28369050.057.7889864
AGTGGTA28432350.057.5931365
GTGGTAT30196300.054.232386
TGGTATC31086800.052.7156687
GGTATCA31228550.052.5420238
GTATCAA31217350.052.530119
CAACGCA31013100.030.30085612-13
ATCAACG31047250.030.29674310-11
AGAGTAC30882800.029.69550118-19
ACGCAGA31844950.029.50165214-15
TACATGG30798000.029.49075922-23
AGTACAT31043700.029.3971320-21
GCAGAGT31890150.029.02959416-17
CATGGGA9441100.027.78066324-25
CATGGGG16686850.027.57859424-25
TCAACGC31208250.026.41018712-13
TATCAAC31346300.026.21794910-11
Produced by FastQC (version 0.11.2)