FastQCFastQC Report
Tue 5 Dec 2017
CBN9DACXX_l08n01.331000000aae31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBN9DACXX_l08n01.331000000aae31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224126693
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACAT7159420.31943629311480537No Hit
GCAGTGGTATCAACGCAGAGTACATGG2544650.1135362310458933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC742850.018.2484551
CCCTCCT899950.018.0325341
CTCTACG868200.017.9635661
CGAGCAC846650.017.9207571
ACCCACT878500.017.6871811
GGTATTG1006700.017.6261791
CGCTTCA618950.017.6209091
ACTGATG759500.017.6063521
CCGGTAG713250.017.5977171
CAATGGA872250.017.5302528
CCGTGGA1020650.017.4923111
TTGGAAC891700.017.46071
TAGTTGG678700.017.4456631
TGACTAG710000.017.4356141
ATGCGAT846700.017.3985981
TTTACTG478900.017.388691
ATTCTAC735800.017.37251
TGGACGC940900.017.3683011
GGGACCT759550.017.311981
CCCATAC749450.017.2761571
Produced by FastQC (version 0.11.2)