FastQCFastQC Report
Tue 5 Dec 2017
CBN9DACXX_l07n03.333000000aae11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBN9DACXX_l07n03.333000000aae11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213157651
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2613330.12260080685539174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT12191050.078.781481
AGCAGTG13537700.070.901112
GCAGTGG18103600.053.0791473
AGTGGTA18169650.052.4870645
CAGTGGT18195600.052.467234
GTGGTAT19577500.048.6944166
TGGTATC20368100.046.8445667
GTATCAA20512500.046.5484289
GGTATCA20658750.046.246058
ATCAACG20337150.027.5038410-11
CAACGCA20353450.027.4642212-13
AGAGTAC20481050.026.74282518-19
ACGCAGA21132900.026.48723814-15
TACATGG20598800.026.38412322-23
AGTACAT20702400.026.34064920-21
GCAGAGT21615300.025.52977616-17
CATGGGA6419900.024.29021624-25
CATGGGG11638450.024.20577224-25
CGACGCT305250.023.6400646
TCAACGC20528800.023.41068512-13
Produced by FastQC (version 0.11.2)