FastQCFastQC Report
Tue 5 Dec 2017
CBN9DACXX_l07n01.331000000aae17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBN9DACXX_l07n01.331000000aae17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213157651
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACAT5417200.25414053751230353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTCA1064200.019.0550581
CAATGGA1252100.018.801248
GTAACTG893150.018.360391
GATGCTA869350.018.3373531
TTAGTTC659500.017.9149041
GGTGCGT1127600.017.8728031
CGCTGGA919350.017.7777251
GTGCTTC678350.017.76711
GACGTGC741750.017.6181011
ATAACGC689100.017.5524371
ATAGACC588850.017.4844741
TTTACTG517950.017.478311
TTGAACG687600.017.4715521
GACTAAC553450.017.4221151
TGGCCAG958950.017.3623981
TCTATTG683700.017.3268931
ATGCGAT808500.017.3044411
AACTCCC725200.017.2567141
GGGAGAT904250.017.1602341
ACGGGTC738950.017.1545561
Produced by FastQC (version 0.11.2)