FastQCFastQC Report
Tue 5 Dec 2017
CBN9DACXX_l06n03.333000000aae04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBN9DACXX_l06n03.333000000aae04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208336665
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA8097960.3886958639757433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT20467600.085.786541
AGCAGTG23201950.075.664462
GCAGTGG30020250.058.510563
CAGTGGT30168400.058.025154
AGTGGTA30295950.057.713055
GTGGTAT32049850.054.5579346
TGGTATC32812250.053.3236277
GGTATCA33047400.052.9989248
GTATCAA33063100.052.9414149
ATCAACG32874350.030.44206410-11
CAACGCA32896950.030.40143412-13
AGAGTAC32783150.029.83630418-19
ACGCAGA33635900.029.72242214-15
TACATGG32637700.029.59989422-23
AGTACAT32936200.029.49749220-21
GCAGAGT33534000.029.46318616-17
CATGGGA10328400.028.28495224-25
CATGGGG16706600.027.6573924-25
TCAACGC33037850.026.59376312-13
TATCAAC33166400.026.43237910-11
Produced by FastQC (version 0.11.2)