FastQCFastQC Report
Tue 5 Dec 2017
CBN9DACXX_l06n01.331000000aae0a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBN9DACXX_l06n01.331000000aae0a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208336665
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACAT6595320.31657029740780385No Hit
GCAGTGGTATCAACGCAGAGTACATGG2499120.11995584166618006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC842250.018.2685221
CTGCGGA778250.017.9514121
CTGCCTA760050.017.8229261
GACTAAC610900.017.7879731
TTGAACG691150.017.7287651
GGCCCTA547600.017.7100110
ACTTTAG584050.017.7012983
GAACCTA795500.017.661181
CGCTATC691400.017.590181
TTTACTG520800.017.5656191
GACTGCG794600.017.5608841
GTCTAGC640850.017.4758058
CGCTTCA521700.017.4265881
CCCTCCT628800.017.3835091
TGCCAAA855450.017.3653011
TGACTAG535700.017.3476451
CCACTAC619900.017.2823221
TGACGGC780700.017.2586571
GTGCTTC595600.017.2237661
CCTTCGA730450.017.1430591
Produced by FastQC (version 0.11.2)