FastQCFastQC Report
Tue 5 Dec 2017
CBN9DACXX_l05n03.333000000aadf6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBN9DACXX_l05n03.333000000aadf6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189591343
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5575020.29405456556104465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT16670200.085.35421
AGCAGTG18997250.074.8886262
GCAGTGG24593750.057.8574183
CAGTGGT24880100.057.004554
AGTGGTA24942950.056.7764475
GTGGTAT26956900.052.5278786
TGGTATC27767100.051.031317
GGTATCA28024250.050.6057328
GTATCAA28065850.050.5131579
ATCAACG27905600.028.9886510-11
CAACGCA27905750.028.97620612-13
AGAGTAC27869250.028.35572618-19
ACGCAGA28601950.028.2790114-15
TACATGG27772000.028.14903322-23
AGTACAT28010750.028.06294820-21
GCAGAGT28452100.028.0603416-17
CATGGGA8612850.026.91524-25
CATGGGG14633100.026.18730524-25
TCAACGC28091450.025.36035512-13
TATCAAC28145650.025.25280210-11
Produced by FastQC (version 0.11.2)