FastQCFastQC Report
Tue 5 Dec 2017
CBN9DACXX_l05n01.331000000aadfc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBN9DACXX_l05n01.331000000aadfc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189591343
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACAT5351190.2822486467644253No Hit
GCAGTGGTATCAACGCAGAGTACATGG2046720.10795429620433672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAG791300.018.5628761
TGAGCCG736050.018.0990981
TGATTTC637200.018.019561
TGGCCAG951900.017.9995631
TGGGCGT837400.017.951
CATATTC552550.017.7547681
TTGAACG688100.017.7432751
CCACCTA742500.017.6772521
CTTTGCG624000.017.6581781
TTTACTG465900.017.6086621
CCTTACG556150.017.6058351
CTGATCC785250.017.571231
AGCTTGA635000.017.5243721
TGAGCAT861250.017.4639051
TGTTCCG650000.017.459421
CGGACTG619400.017.4517521
CGTGAGC535900.017.3182371
TTACGCG455900.017.3059379
ACTGCTC613250.017.2926651
ACTGATG485950.017.20431
Produced by FastQC (version 0.11.2)