FastQCFastQC Report
Tue 5 Dec 2017
CBN9DACXX_l04n03.333000000aade9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBN9DACXX_l04n03.333000000aade9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180575707
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2799440.15502860525973186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT12808050.081.57881
AGCAGTG14015950.074.53362
GCAGTGG19269250.054.280433
CAGTGGT19245550.054.1387834
AGTGGTA19495950.053.366645
GTGGTAT21554100.048.179896
TGGTATC22228550.046.7454267
GTATCAA22467200.046.358769
GGTATCA22514450.046.2850158
ATCAACG22452100.026.68849410-11
CAACGCA22491400.026.67179312-13
ACGCAGA23177600.025.91180214-15
TACATGG22384450.025.84727122-23
AGTACAT22649300.025.69588720-21
AGAGTAC22888750.025.5705918-19
GCAGAGT23637850.025.01213516-17
CATGGGA6628900.024.55896824-25
CATGGGG12857650.024.01905424-25
TCAACGC22599050.023.145112-13
AACGCAG23028350.022.77705614-15
Produced by FastQC (version 0.11.2)