FastQCFastQC Report
Tue 5 Dec 2017
CBN9DACXX_l04n01.331000000aadef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBN9DACXX_l04n01.331000000aadef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180575707
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACAT3316090.18363987355176187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGTA982400.018.9037444
CCACCTA1170650.018.8116931
GGAGCAA920400.018.5460551
ACGGAGA1100100.018.5436941
TTCGTAG623850.018.5303295
GTCGAAC769600.018.3399228
GGAGCGA640150.018.2385129
CCAGCGA819200.018.1762391
AGGAGCG650700.018.1687058
TGGGCGT767400.018.086241
GGAGATC617450.018.0384273
TCGAATG727050.017.9922438
CATGGCG983500.017.9607321
TTTACTG458450.017.9374371
AGTCGTA522500.017.932458
AGTGACC539250.017.91669710
AGAGTGA864950.017.905938
ACGAGCC625250.017.8700681
GCAGCTC611850.017.7658983
CAGATCA1220050.017.756391
Produced by FastQC (version 0.11.2)