FastQCFastQC Report
Tue 5 Dec 2017
CBN9DACXX_l03n03.333000000aadb2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBN9DACXX_l03n03.333000000aadb2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188301581
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4361550.2316257769497963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT16609050.083.805461
AGCAGTG18665650.074.571152
GCAGTGG24542450.056.7311173
CAGTGGT24667750.056.2330064
AGTGGTA24963150.055.5106545
GTGGTAT27149200.051.056316
TGGTATC27750600.049.921317
GGTATCA28071800.049.3618668
GTATCAA28051600.049.355899
ATCAACG28010250.028.62205510-11
CAACGCA28015750.028.60769712-13
ACGCAGA28730700.027.92650814-15
TACATGG27863800.027.84727722-23
AGAGTAC28271750.027.77797918-19
AGTACAT28194900.027.67644520-21
GCAGAGT29280350.027.09129316-17
CATGGGA8311850.026.54539124-25
CATGGGG14852250.026.04393224-25
TCAACGC28135200.024.72070312-13
AACGCAG28603650.024.33874914-15
Produced by FastQC (version 0.11.2)