FastQCFastQC Report
Tue 5 Dec 2017
CBN9DACXX_l03n01.331000000aadb8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBN9DACXX_l03n01.331000000aadb8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188301581
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACAT4256420.2260427117709649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACTC1002150.018.6493361
CCTATTA755650.018.1906721
CCTTACG655750.018.1488461
TGACTAG723100.018.0855521
CCAGCGA777250.017.9257261
CGACCTT798900.017.8922791
GATGCTA614750.017.8179021
CCGTGGA721000.017.7040421
CTTTGCG701750.017.5819191
GTCGTAA634550.017.5665381
CGCTATC550650.017.5397971
GATCGCG586850.017.5300981
ATAACGC634750.017.4881841
GACCTGG629800.017.4421581
CCCTCCT605650.017.4056951
GGATTAC600800.017.3853421
GCAGTTA491000.017.3749561
CCGGTAG501200.017.3043121
CGCTTTC540350.017.24319310
ACGAGCC552700.017.216251
Produced by FastQC (version 0.11.2)