FastQCFastQC Report
Tue 5 Dec 2017
CBN9DACXX_l02n03.333000000aadcf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBN9DACXX_l02n03.333000000aadcf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204341248
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4986170.24401191872920341No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGATAAAATTTGAAATCTG2220840.10868290282733323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT15636900.083.055241
AGCAGTG17651200.073.574542
GCAGTGG23271850.055.8296623
CAGTGGT23354500.055.413524
AGTGGTA23516450.054.960955
GTGGTAT25526550.050.6287576
TGGTATC26181300.049.4049077
GGTATCA26458400.048.938658
GTATCAA26456750.048.9383629
ATCAACG26360400.028.34216910-11
CAACGCA26380850.028.31402812-13
TACATGG26185700.027.51781822-23
ACGCAGA27154700.027.50039514-15
AGAGTAC26585400.027.37160518-19
AGTACAT26550750.027.2400220-21
GCAGAGT27290450.026.92210616-17
CATGGGA8039100.025.81515724-25
CATGGGG13619000.025.40282624-25
TCAACGC26506750.024.53633712-13
GAGTACA26176400.024.15458120-21
Produced by FastQC (version 0.11.2)