FastQCFastQC Report
Tue 5 Dec 2017
CBN9DACXX_l02n01.331000000aadc5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBN9DACXX_l02n01.331000000aadc5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204341248
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACAT5174930.2532494075792274No Hit
GCAGTGGTATCAACGCAGAGTACATGG2044260.10004147571810856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC832950.018.81071
CATATTC811950.018.7046871
AGAGCGA780550.018.2499851
TGAGCCG869350.018.1535851
CTGCGGA834000.018.1005761
GATCTAG737850.018.004911
TCGAATG753800.017.9460378
ACGAGCC822450.017.9281851
GGGAATG811900.017.9010931
TACTTAC556250.017.8400231
TTGAACG690750.017.8107281
CACCGAA730800.017.7881688
CCTACCA723250.017.7293171
CGCTATC641650.017.6233041
ATCGAGT872200.017.6016851
CTGATCC558750.017.5797211
CGGCTAG626150.017.49421
AAGACCT704800.017.46761
CCGCCTA552750.017.34453410
GACGTGC667850.017.2779671
Produced by FastQC (version 0.11.2)