FastQCFastQC Report
Tue 5 Dec 2017
CBN9DACXX_l01n03.333000000aada6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBN9DACXX_l01n03.333000000aada6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176557749
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4119940.233348013515963No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGATAAAATTTGAAATCTG2965890.1679841307899774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT14981300.084.8557741
AGCAGTG17204900.073.866252
GCAGTGG22647550.056.1538773
CAGTGGT22690450.055.84214
AGTGGTA22945050.055.1798745
GTGGTAT24687550.051.2740556
TGGTATC25154250.050.3336877
GGTATCA25474100.049.8854688
GTATCAA25541500.049.7499129
ATCAACG25453500.028.9810610-11
CAACGCA25527800.028.89013512-13
AGAGTAC25784000.028.11327618-19
ACGCAGA26282450.028.07020814-15
TACATGG25498350.028.04803522-23
GCAGAGT26231150.027.84510216-17
AGTACAT25896850.027.78821620-21
CATGGGG12981500.026.13523124-25
CATGGGA7834500.026.12704324-25
TCAACGC25631350.024.90402812-13
GAGTACA25531350.024.52754820-21
Produced by FastQC (version 0.11.2)