FastQCFastQC Report
Tue 5 Dec 2017
CBN9DACXX_l01n01.331000000aadac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBN9DACXX_l01n01.331000000aadac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176557749
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACAT3820960.21641417732393042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATCC637250.018.5103451
GGATTAC766750.018.4134481
GACTAAC553100.018.238121
CCCTCCT754850.018.2319281
TGATTTC700000.018.0981941
CTAAGAC595350.018.0890521
TGGGCGT664450.017.7494431
TTTGCGC621800.017.5392271
AGGGATG726500.017.3816431
CTGCCTA621150.017.1770341
GATCTAG511300.017.1381631
CATATTC474350.017.1354561
TAAACCG620600.017.0517521
AGCTTGA585850.017.0141471
ACTGAAC587150.016.9836331
TCTTGAG403550.016.9369588
TCGCGAG559600.016.9317821
CCTCTGA571500.016.915621
TGGCCAG629900.016.8817211
TGACGGC602150.016.8676261
Produced by FastQC (version 0.11.2)