FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l08_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l08_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13111639
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT5400.025.0195182
GTAGATC3450.023.47642732
TCTCGGT3450.022.82360837
GTGTAGA3450.022.1721830
TAGATCT3650.021.5736633
AGTGTAG3700.020.06629
GAGTGTA3700.020.06454728
TGTAGAT4050.019.44292631
TCCCTAC3200.018.2795459
AACCGCT2400.017.8111131
CTCGGTG5350.017.66163338
TGTAGGG4350.017.58543818
GTGTAGG4700.017.23210517
GATCTCG5250.016.7129835
GTAGGGA4600.016.62983319
TCGTGTA4600.016.62888315
TAATCCG952.824584E-416.57708417
TAGGGAA5050.015.59349620
TCGGAAG9200.015.1747653
AGATCTC5550.014.99882934
Produced by FastQC (version 0.11.5)