FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l08_n02_S1W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l08_n02_S1W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33897302
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC46950.011.02136511
ACTTTCG34250.010.44489419
TTAAGTC59950.010.1414422
GGTAAGT48400.010.04004117
AGTCTCG56200.010.0153035
TAAGTCT57600.010.0077893
GTACTCG53900.09.482944
CGCGATA27850.09.45169229
GTAAGTT52650.09.35860918
CGAAATT61150.09.2059522
GGAAAAT148950.08.9043431
CGATAGT30750.08.70664331
CGTAACG55700.08.68364442
TAGAACA61600.08.58282512
GTTCGAT86400.08.5484461
TTCGATC76350.08.523422
ACATGCG51000.08.4710586
GCGTAAC56100.08.46132541
TAACGAT58600.08.40738344
GGGGAAT100900.08.3242431
Produced by FastQC (version 0.11.5)