FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l08_n02_S1W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l08_n02_S1W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28429907
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC46350.012.42610211
TAGAACA61100.09.46316912
GGTAGAA61700.09.33468110
TCAGGTA65500.09.3090537
CTAATCG32950.09.0890813
CATAAGC95800.09.04217157
TAATCGA38250.09.0067574
GGAAAAT145200.08.9329191
AAAATCC101450.08.92272953
CAGGTAG68200.08.5439778
TTACGAC35950.08.51121529
AGCATAA101000.08.4932035
GGTAAGT26100.08.44709417
AATCCTT92500.08.39740855
CGAAATT41350.08.2775192
AATTCAC43100.08.1492055
AATCGAA55400.08.1280835
AAATCCT108350.08.1048344
AGTGTGA76600.07.87137714
ATAAGCA149050.07.84903148