FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l08_n02_S1W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l08_n02_S1W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23105928
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG480930.208141391248168Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG67950.034.40017716
GAGTGTA71650.032.1822928
AGTGTAG74950.030.73836729
TCGTGTA76700.030.59363415
TGTAGGG77400.030.43506218
GTGTAGG78800.029.89123317
GTAGATC79550.029.47005532
TCTCGGT79450.029.44872937
GTGTAGA83450.027.9309630
TAGATCT84800.027.83127233
ATCTCGG86750.027.4637136
GTAGGGA86400.027.36908319
TAGGGAA87950.027.11697820
GTCGTGT87950.026.96173714
ATCGGAA93150.026.7839833
GAGCGTC96200.025.46811710
TCGGAAG96450.024.9095214
AGAGCGT99250.024.481519
TGTAGAT97350.024.15087331
AGCGTCG103700.023.08372911
Produced by FastQC (version 0.11.5)